Exploring the regulation of the COP9signalosome/NEDD8 axis by oxidative stress
Increased oxygen-derived reactive species (ROS) levels induce progressive damage to macromolecules, cells, and tissues, eventually leading to diseases such as cancer, inflammation, neurodegeneration, and cardiovascular disorders. Most aerobic organisms sense increased ROS concentrations and respond to protect cellular redox homeostasis through a defense mechanism, including transcriptional modulation and activation of ROS scavengers.
Our lab interest is to determine the effect of elevated ROS levels on cullin NEDDylation and deNEDDylation enzymes and to investigate the ROS defense mechanism underlying the accumulated free NEDD8.
The research project proposed herein is located at the laboratory of Dr. Elah Pick, at the unique Oranim Biology off-campus of the University of Haifa in Tivon, Israel. The project aims to investigate the molecular mechanism responsible for sensitivity of the NEDDylation pathway to oxidative stress, and the biochemical and cellular function behind it.
We are looking for highly motivated PhD and postdoctoral fellows with knowledge in one or more of these areas:
- protein biochemistry
- the ubiquitin-proteasome pathway
- approaching budding yeast or other fungi as a model system
- mammalian tissue culture