Project title: Insights into the role of DNA break processing factors in maintaining genome stability to target cancer cells
Beginning and duration: April/June 2018 - 1 year, renewable
Description: The ability of cells to detect and properly repair double stranded DNA breaks (DSBs) is essential for maintaining genome stability and preventing cancer development.
Indeed, DSBs are the most cytotoxic forms of DNA damage, because inaccurate DSB repair leads to mutations and/or gross chromosomal rearrangements. A critical step in regulating DSB repair is the processing of its DNA ends. Several factors are involved in this mechanism, which are conserved in all eukaryotes.
Mutations in most of these factors lead to genome instability, cancer and severe human inherited diseases. The candidate will characterize the functional role of some of these factors in human cell lines, in term of DNA repair and checkpoint response.
The CAS9-based technology will be used by the candidate to create DSBs in defined sites in the genome of different human patient derived cell lines. The processing and repair of CAS9-induced DSB will be analysed with standard and innovative techniques.
Requirements: Good theoretical knowledge and possibly practical experience in genetics, biochemistry, molecular and cell biology. Experience in mammalian cell culturing. High motivation and dedication. Good knowledge of English. At least one paper published in a major international journal. Previous experience with DNA damage response and cell cycle is a plus.
Reference letters required.
Contact: Achille Pellicioli,
Dipartimento di Bioscienze
Università degli Studi di Milano
Via Celoria 26, 20133 MILANO
Ph: 02 50315033